Mouse anti-Human Filaggrin

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (152274651..152297679, complement). Location: 1q21.3

Monoclonal

IgG1,k

5G114

Mouse

Recognizes human Filaggrin.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide

0.2 mg/ml (lot specific)

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-FLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Filaggrins are an important class of the intermediate filament-associated proteins which interect with keratin intermediate filaments (Ifs) of terminally differentiating mammalian epidermis. Filaggrins exhibit wide species variations. Filaggrin also appears to be a target molecule for rheumatoid arthritis-specific auto-antibodies in humans. Filaggrin is expressed only in well differemtiated keratinized epithelial cells. The synthesis of filaggrin varies in types of ichthyosis, and with viral, premalignant and malignant conditions.

Antibodies; Abs to Proteins

Immunohistochemistry (IHC)
Other applications not tested

Recommended Dilution:
Immunohistology (FFPE): (2-4ug/ml for 30 min at RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human skin stained with MBS603861 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of keratinized epithelial cells of hair follicle.

38kD (Filaggrin) and 400kD (Profilaggrin)[Similar Products]

filaggrin

filaggrin; OTTHUMP00000014057; epidermal filaggrin

Filaggrin

FILA_HUMAN

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus

Molecular Function: protein binding; calcium ion binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2

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