Product Name
GLE1, Polyclonal Antibody
Popular Item
Full Product Name
GLE1 Polyclonal Antibody
Product Synonym Names
GLE1L; hGLE1; LCCS; LCCS1
Product Gene Name
anti-GLE1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
MPSEGRCWET LKALRSSDKG RLCYYRDWLL RREDVLEECM SLPKLSSYSG WVVEHVLPHM QENQPLSETS PSSTSASALD QPSFVPKSPD ASSAFSPASP ATPNGTKGKD ESQHTESMVL QSSRGIKVEG CVRMYELVHR MKGTEGLRLW QEEQERKVQA LSEMASEQLK RFDEWKELKQ HKEFQDLREV MEKSSREALG HQEKLKAEHR HRAKILNLKL REAEQQRVKQ AEQERLRKEE GQIRLRALYA LQEEM
3D Structure
ModBase 3D Structure for Q53GS7
Species Reactivity
Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human GLE1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, Cytoplasm, Nucleus, nuclear pore complex
Positive Samples
Mouse spleen, Mouse liver
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-GLE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GLE1 antibody
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-GLE1 antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-GLE1 antibody
Western Blot (WB)
Application Notes for anti-GLE1 antibody
WB: 1:1000 - 1:2000
Western Blot (WB) of anti-GLE1 antibody
Western blot analysis of extracts of various cell lines, using GLE1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
NCBI/Uniprot data below describe general gene information for GLE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001003722.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001003722.1
[Other Products]
UniProt Primary Accession #
Q53GS7
[Other Products]
UniProt Secondary Accession #
O75458; Q53GT9; Q5VVU1; Q8NCP6; Q9UFL6[Other Products]
UniProt Related Accession #
Q53GS7[Other Products]
Molecular Weight
Calculated: 75kDa; 79kDa
Observed: 80kDa
NCBI Official Full Name
nucleoporin GLE1 isoform 1
NCBI Official Synonym Full Names
GLE1, RNA export mediator
NCBI Official Symbol
GLE1??[Similar Products]
NCBI Official Synonym Symbols
LCCS; GLE1L; LCCS1; hGLE1
??[Similar Products]
NCBI Protein Information
nucleoporin GLE1
UniProt Protein Name
Nucleoporin GLE1
UniProt Synonym Protein Names
GLE1-like protein
UniProt Gene Name
GLE1??[Similar Products]
UniProt Synonym Gene Names
GLE1L; hGLE1??[Similar Products]
NCBI Summary for GLE1
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GLE1
Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC).
Research Articles on GLE1
1. C mutation generates a protein isoform capable of both Gle1A- and Gle1B-ascribed functions, and thereby uncoupled from normal mechanisms of Gle1 regulation.">It was concluded that the amyotrophic lateral sclerosis-linked Gle1-c.1965-2A>C mutation generates a protein isoform capable of both Gle1A- and Gle1B-ascribed functions, and thereby uncoupled from normal mechanisms of Gle1 regulation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
