Product Name
IFNGR1, siRNA
Full Product Name
IFNGR1 siRNA (Mouse)
Product Synonym Names
IFNGR; Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; CD antigen CD119
Product Gene Name
IFNGR1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15261
Specificity
IFNGR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IFNGR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IFNGR1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IFNGR1 sirna
siRNA to inhibit IFNGR1 expression using RNA interference
Applications Tested/Suitable for IFNGR1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IFNGR1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034641.1
[Other Products]
NCBI GenBank Nucleotide #
NM_010511.2
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UniProt Primary Accession #
P15261
[Other Products]
UniProt Secondary Accession #
Q91Y85[Other Products]
UniProt Related Accession #
P15261[Other Products]
Molecular Weight
52,343 Da
NCBI Official Full Name
interferon gamma receptor 1
NCBI Official Synonym Full Names
interferon gamma receptor 1
NCBI Official Symbol
Ifngr1??[Similar Products]
NCBI Official Synonym Symbols
Ifgr; CD119; Ifngr; Nktar; IFN-gammaR
??[Similar Products]
NCBI Protein Information
interferon gamma receptor 1
UniProt Protein Name
Interferon gamma receptor 1
UniProt Synonym Protein Names
CD_antigen: CD119
Protein Family
Interferon gamma receptor
UniProt Gene Name
Ifngr1??[Similar Products]
UniProt Synonym Gene Names
Ifngr; IFN-gamma receptor 1; IFN-gamma-R1??[Similar Products]
UniProt Entry Name
INGR1_MOUSE
UniProt Comments for IFNGR1
IFNGR1: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type II cytokine receptor family.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: membrane; endoplasmic reticulum; dendrite; postsynaptic density; integral to membrane; plasma membrane; vesicle
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; cytokine binding
Biological Process: cytokine and chemokine mediated signaling pathway
Research Articles on IFNGR1
1. These data indicate that both GKO and GRKO mice fail to develop an IFN-gamma-mediated antiviral response, but differ in regulation of the inflammatory response to infection.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
