Product Name
NKX2-1, siRNA
Full Product Name
NKX2-1 siRNA (Mouse)
Product Synonym Names
NKX-2.1; TITF1; TTF1; Homeobox protein Nkx-2.1; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1
Product Gene Name
NKX2-1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50220
Specificity
NKX2-1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NKX2-1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NKX2-1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NKX2-1 sirna
siRNA to inhibit NKX2-1 expression using RNA interference
Applications Tested/Suitable for NKX2-1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NKX2-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001139670.1
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NCBI GenBank Nucleotide #
NM_001146198.1
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UniProt Primary Accession #
P50220
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UniProt Related Accession #
P50220[Other Products]
Molecular Weight
38,570 Da
NCBI Official Full Name
homeobox protein Nkx-2.1
NCBI Official Synonym Full Names
NK2 homeobox 1
NCBI Official Symbol
Nkx2-1??[Similar Products]
NCBI Official Synonym Symbols
T/EBP; Titf1; Ttf-1; Nkx2.1; AV026640
??[Similar Products]
NCBI Protein Information
homeobox protein Nkx-2.1
UniProt Protein Name
Homeobox protein Nkx-2.1
UniProt Synonym Protein Names
Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1; Thyroid-specific enhancer-binding protein; T/EBP
Protein Family
Homeobox protein
UniProt Gene Name
Nkx2-1??[Similar Products]
UniProt Synonym Gene Names
TTF-1; T/EBP??[Similar Products]
UniProt Entry Name
NKX21_MOUSE
UniProt Comments for NKX2-1
NKX2-1: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC); also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in *****hood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). This syndrome include neurological, thyroid, and respiratory problems. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; axon guidance; positive regulation of transcription, DNA-dependent; rhythmic process; neuron migration; globus pallidus development; response to lipopolysaccharide; locomotory behavior; forebrain dorsal/ventral pattern formation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of circadian rhythm; forebrain neuron differentiation; cerebral cortex neuron differentiation; Leydig cell differentiation; hyperosmotic salinity response; regulation of transcription, DNA-dependent; phospholipid metabolic process; renin-angiotensin regulation of blood volume; thyroid gland development; oligodendrocyte differentiation; anatomical structure formation; feeding behavior; forebrain neuron fate commitment; negative regulation of cell migration; transcription, DNA-dependent; hippocampus development; induction; neuron fate commitment; cerebral cortex GABAergic interneuron differentiation; pattern specification process; menarche; regulation of transcription from RNA polymerase II promoter; telencephalon development; organ morphogenesis; cerebral cortex cell migration; pituitary gland development; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; lung development; endoderm development; telencephalon cell migration
Research Articles on NKX2-1
1. Prototypic GPe neurons derive from the medial ganglionic eminence of the embryonic subpallium and express the transcription factor Nkx2-1. These neurons fire at high rates during alert rest, and encode movements through heterogeneous firing rate changes.
Precautions
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Disclaimer
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