MUSK (Muscle Skeletal Receptor Tyrosine Kinase)/Muscle-specific Receptor Tyrosine Kinase/muscle; skeletal; receptor tyrosine kinase/skeletal receptor tyrosine-protein kinase

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MUSK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760450 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Muscle, skeletal receptor tyrosine-protein kinase (MUSK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MUSK. The ELISA analytical biochemical technique of the MBS760450 kit is based on MUSK antibody-MUSK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUSK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUSK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MUSK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MUSK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MUSK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MUSK can be calculated.

86,425 Da

muscle, skeletal, receptor tyrosine kinase

muscle, skeletal receptor tyrosine-protein kinase

Muscle, skeletal receptor tyrosine-protein kinase

MuSK; Muscle-specific kinase receptor??[Similar Products]

MUSK_HUMAN

This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Musk family

Chromosomal Location of Human Ortholog: 9q31.3-q32

Cellular Component: cell junction; integral to plasma membrane; neuromuscular junction; postsynaptic membrane; receptor complex

Molecular Function: ATP binding; metal ion binding; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell differentiation; extracellular matrix organization and biogenesis; memory; multicellular organismal development; neuromuscular junction development; peptidyl-tyrosine phosphorylation; positive regulation of neuron apoptosis; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; regulation of synaptic growth at neuromuscular junction; regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Fetal Akinesia Deformation Sequence; Myasthenic Syndrome, Congenital, 9, Associated With Acetylcholine Receptor Deficiency

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.