Homeobox protein Nkx-21; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1; Thyroid-specific enhancer-binding protein; T/EBP; NKX2-1; NKX2A; TITF1; TTF1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-NKX2-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a protein initially identified as a
thyroid-specific transcription factor. The encoded protein binds to
thyroglobulin promoter and regulates the expression of
thyroid-specific genes but has also been shown to regulate the
expression of genes involved in morphogenesis. Mutations and
deletions in this gene are associated with benign hereditary
chorea, choreoathetosis, congenital hypothyroidism, and neonatal
respiratory distress, and may be associated with thyroid cancer.
Multiple transcript variants encoding different isoforms have been
found for this gene.

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

WB~~1:1000

NKX2-1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NKX2-1 antibody detected the NKX2-1 protein (arrow).

NKX2-1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

38596

NK2 homeobox 1

homeobox protein Nkx-2.1

Homeobox protein Nkx-2.1

TTF-1; T/EBP??[Similar Products]

NKX21_HUMAN

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]

NKX2-1: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC); also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in *****hood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). This syndrome include neurological, thyroid, and respiratory problems. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; axon guidance; positive regulation of transcription, DNA-dependent; neuron migration; response to hormone stimulus; rhythmic process; globus pallidus development; forebrain dorsal/ventral pattern formation; locomotory behavior; positive regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; Leydig cell differentiation; phospholipid metabolic process; forebrain development; thyroid gland development; oligodendrocyte differentiation; anatomical structure formation; forebrain neuron fate commitment; negative regulation of cell migration; hippocampus development; induction; cerebral cortex GABAergic interneuron differentiation; cerebral cortex cell migration; pituitary gland development; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development; endoderm development

Disease: Thyroid Carcinoma, Papillary; Chorea, Benign Hereditary; Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction

Kim, J.H., et al. Acta Cytol. 54(3):277-282(2010)
Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010)
Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010)
Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010) :
Cantara, S., et al. Thyroid Res 3 (1), 4 (2010) :

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