Human PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP) ELISA kit; MGC161555; PHLPP; PLEKHE1; SCOP; PH domain and leucine rich repeat protein phosphatase; SCN circadian oscillatory protein; pleckstrin homology domain containing; family E (with leucine rich repeats) m; PH domain and leucine rich repeat protein phosphatase

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

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Small volumes of PHLPP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9331362 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PH domain and leucine rich repeat protein phosphatase (PHLPP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PHLPP. The ELISA analytical biochemical technique of the MBS9331362 kit is based on PHLPP antibody-PHLPP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PHLPP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PHLPP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PHLPP (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

184,672 Da

PH domain and leucine rich repeat protein phosphatase 1

PH domain leucine-rich repeat-containing protein phosphatase 1; SCN circadian oscillatory protein; PH domain-containing family E member 1; suprachiasmatic nucleus circadian oscillatory protein; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1

PH domain leucine-rich repeat-containing protein phosphatase 1

KIAA0606; PHLPP; PLEKHE1; SCOP; PH domain-containing family E member 1; hSCOP??[Similar Products]

PHLP1_HUMAN

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]

PHLPP: a pleckstrin homology domain leucine-rich repeat protein phosphoserine and ??threonine phosphatase. A tumor suppressor that controls the onset and progression of various cancers because it controls the balance between cell survival and apoptosis. Catalyzes the dephosphorylation of the hydrophobic motif on the AGC kinases Akt, PKC, and S6 kinase, and the inhibitory site on the kinase Mst1. The dephosphorylation of PKCA and PKCB is destabilizing, leading to its degradation. The dephosphorylation of p70S6K at T390 reduces its activity, thus reducing the efficiency of protein translation. Dephosphorylation of Mst1 by PHLPP of an autoinhibitory site (T387) activates Mst1, resulting in the activation of the pro-apoptotic MAPK pathway. Ubiquitously expressed in non-cancerous tissues. Insensitive to inhibition by okadaic acid. PHLPP loss occurs at high frequency in colorectal cancer, and leads to the development of prostate tumors in mice. PHLPP degradation by the 26 S proteasome is controlled by a beta-TrCP-containing Skp??Cullin 1??F-box protein (SCF) complex E3 ubiquitin ligase in a phosphorylation-dependent manner. This degradation requires phosphorylation of PHLPP1 by CK I and GSK-3beta. Activation of the PI3K/Akt pathway suppresses the degradation of PHLPP1 by inhibiting GSK-3beta activity. PHLPP2 activity is highly sensitive to a common polymorphism (L-1016-S) that reduces its rate of dephosphorylation of the hydrophobic motif of Akt and PKC, rendering it ineffective at inducing apoptosis. This variant is expressed by 30% of the human population. Interacts with the nucleotide free form of KRas via its LRR repeats. Interacts with AKT2, AKT3 and PKCB isoform beta-II.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Tumor suppressor

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: membrane; nucleus; cytosol

Molecular Function: protein binding; metal ion binding; phosphoprotein phosphatase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; dephosphorylation; nerve growth factor receptor signaling pathway; apoptosis; innate immune response; entrainment of circadian clock

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