Full Product Name
SOX3 siRNA (Human)
Product Synonym Names
Transcription factor SOX-3
Product Gene Name
SOX3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P41225
Specificity
SOX3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SOX3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SOX3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SOX3 sirna
siRNA to inhibit SOX3 expression using RNA interference
Applications Tested/Suitable for SOX3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SOX3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005625.2
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NCBI GenBank Nucleotide #
NM_005634.2
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UniProt Primary Accession #
P41225
[Other Products]
UniProt Secondary Accession #
P35714; Q5JWI3; Q9NP49[Other Products]
UniProt Related Accession #
P41225[Other Products]
Molecular Weight
45,210 Da
NCBI Official Full Name
transcription factor SOX-3
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 3
NCBI Official Symbol
SOX3??[Similar Products]
NCBI Official Synonym Symbols
PHP; GHDX; MRGH; PHPX; SOXB
??[Similar Products]
NCBI Protein Information
transcription factor SOX-3
UniProt Protein Name
Transcription factor SOX-3
Protein Family
Transcription factor
UniProt Gene Name
SOX3??[Similar Products]
UniProt Entry Name
SOX3_HUMAN
NCBI Summary for SOX3
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for SOX3
SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: Xq27.1
Cellular Component: nucleus
Molecular Function: DNA binding; transcription factor activity
Biological Process: sex determination; spermatid differentiation; organ morphogenesis; hypothalamus development; negative regulation of neuron differentiation; sensory organ development; central nervous system development; transcription, DNA-dependent; pituitary gland development; neuron development; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development
Disease: Panhypopituitarism, X-linked; Mental Retardation, X-linked, With Panhypopituitarism
Research Articles on SOX3
1. Our study provides additional evidence that deletion in polyalanine tracts of SOX3 is associated with hypopituitarism
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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