Product Name
CFL2 / Cofilin 2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human CFL2 / Cofilin 2
Product Synonym Names
Anti-CFL2 / Cofilin 2 Antibody (C-Terminus) IHC-plus; CFL2; Cofilin 2; Cofilin-2; NEM7; Cofilin 2 (muscle); Cofilin; muscle isoform; Human CFL2; Cofilin 2
Product Gene Name
anti-CFL2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y281
Species Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat
Specificity
Human CFL2. This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide C-DIKDRSTLGEK from the C-terminus of human CFL2 (NP_068733.1; NP_619579.1).
Immunogen Type
Synthetic peptide
Immunogen
CFL2 / Cofilin 2 antibody was raised against synthetic peptide C-DIKDRSTLGEK from the C-terminus of human CFL2 (NP_068733.1; NP_619579.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%); Baboon, Lizard (91%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-CFL2 antibody
Family: Cytoskeleton
Applications Tested/Suitable for anti-CFL2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CFL2 antibody
ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.01 - 0.03 ug/ml)
Immunohistochemistry (IHC) of anti-CFL2 antibody
Anti-CFL2 / Cofilin 2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western Blot (WB) of anti-CFL2 antibody
(0.003 ug/ml) Staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for CFL2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_619579.1
[Other Products]
NCBI GenBank Nucleotide #
NM_138638.4
[Other Products]
UniProt Primary Accession #
Q9Y281
[Other Products]
UniProt Secondary Accession #
G3V5P4[Other Products]
UniProt Related Accession #
Q9Y281[Other Products]
NCBI Official Full Name
cofilin-2 isoform 1
NCBI Official Synonym Full Names
cofilin 2 (muscle)
NCBI Official Symbol
CFL2??[Similar Products]
NCBI Official Synonym Symbols
NEM7
??[Similar Products]
NCBI Protein Information
cofilin-2; cofilin, muscle isoform; nemaline myopathy type 7
UniProt Protein Name
Cofilin-2
UniProt Synonym Protein Names
Cofilin, muscle isoform
UniProt Gene Name
CFL2??[Similar Products]
UniProt Entry Name
COF2_HUMAN
NCBI Summary for CFL2
This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Comments for CFL2
Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
Protein type: Nuclear receptor co-regulator; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton
Molecular Function: protein binding; actin binding
Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization
Disease: Nemaline Myopathy 7
Research Articles on CFL2
1. patients with severe nemaline myopathy should be screened for mutations in CFL2.
Precautions
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Disclaimer
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