NEM7

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 98 % as determined by SDS-PAGE

Lyophilized from sterile PBS, pH 7.5

Samples are stable for up to twelve months from date of receipt at -70 degree C

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of CFL2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.?

Description: A DNA sequence encoding the human CFL2 (Q9Y281-1) (Ala 2-Leu 166) was expressed, with a polyhistidine tag at the N-terminus.

The recombinant human CFL2 consisting of 180 amino acids and has a calculated molecular mass of 20.4 kDa. The apparent molecular mass of the protein is approximately 21 kDa in SDS-PAGE under reducing conditions.

a?? Da

cofilin 2

cofilin-2

Cofilin-2

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: extracellular space; I band; Z disc

Molecular Function: actin filament binding; protein binding

Biological Process: actin filament depolymerization; positive regulation of actin filament depolymerization

Disease: Nemaline Myopathy 7

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