Product Name
Cofilin 2 (CFL2), Polyclonal Antibody
Full Product Name
Cofilin 2 antibody
Product Gene Name
anti-CFL2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 127 of Cofilin 2
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CFL2 antibody
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]
Product Categories/Family for anti-CFL2 antibody
Total protein Ab
Applications Tested/Suitable for anti-CFL2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-CFL2 antibody
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Testing Data of anti-CFL2 antibody
Sample (30 ug of whole cell lysate) A: H1299 B: Molt-4 12% SDS PAGE Primary antibody diluted at 1: 1000
Immunofluorescence (IF) of anti-CFL2 antibody
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2500.
NCBI/Uniprot data below describe general gene information for CFL2. It may not necessarily be applicable to this product.
NCBI Accession #
AAH11444
[Other Products]
UniProt Secondary Accession #
G3V5P4[Other Products]
UniProt Related Accession #
Q9Y281[Other Products]
Molecular Weight
– Da[Similar Products]
NCBI Official Full Name
Cofilin 2 (muscle)
NCBI Official Synonym Full Names
cofilin 2 (muscle)
NCBI Official Symbol
CFL2??[Similar Products]
NCBI Official Synonym Symbols
NEM7
??[Similar Products]
NCBI Protein Information
cofilin-2
UniProt Protein Name
Cofilin-2
UniProt Synonym Protein Names
Cofilin, muscle isoform
UniProt Gene Name
CFL2??[Similar Products]
UniProt Entry Name
COF2_HUMAN
NCBI Summary for CFL2
This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Comments for CFL2
Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton
Molecular Function: protein binding; actin binding
Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization
Disease: Nemaline Myopathy 7
Research Articles on CFL2
1. Cofilin 2 phosphorylation and genetic overexpression plays a role in the pathogenesis of idiopathic dilated cardiomyopthay.
Precautions
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Disclaimer
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