For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human MOV10. No significant cross-reactivity or interference between Human MOV10 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MOV10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281971 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Putative helicase MOV-10 (MOV10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MOV10. The ELISA analytical biochemical technique of the MBS281971 kit is based on MOV10 antibody-MOV10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MOV10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MOV10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MOV10 in samples. An antibody specific for MOV10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOV10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOV10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOV10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6,546 Da

Mov10 RISC complex RNA helicase

putative helicase MOV-10

Putative helicase MOV-10

KIAA1631??[Similar Products]

MOV10: Probable RNA helicase. Required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA- mediated cleavage of complementary mRNAs by RISC. Also required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription. Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase; RNA processing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytosol; extracellular space

Molecular Function: ATP binding; helicase activity; protein binding; RNA binding

Biological Process: miRNA-mediated gene silencing; mRNA cleavage involved in gene silencing by miRNA; phosphoinositide-mediated signaling; regulation of megakaryocyte differentiation; regulation of transcription, DNA-templated; transcription, DNA-dependent; Wnt receptor signaling pathway, calcium modulating pathway

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.