Product Name
Cofilin 2 (CFL2), Polyclonal Antibody
Full Product Name
Goat anti-Cofilin 2 (muscle) Antibody
Product Synonym Names
cofilin 2 (muscle); HGNC: 1875; CFL2; NEM7; cofilin 2; cofilin 2 (muscle) antibody; HGNC: 1875 antibody; CFL2 antibody; NEM7 antibody; cofilin 2 antibody; Cofilin 2; Cofilin 2 (muscle)
Product Gene Name
anti-CFL2 antibody
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Antibody/Peptide Pairs
Cofilin 2 peptide (MBS426916) is used for blocking the activity of Cofilin 2 antibody (MBS420805)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DIKDRSTLGE K
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DIKDRSTLGEK, from the C Terminus of the protein sequence according to NP_068733.1; NP_619579.1.
Note
This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CFL2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CFL2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 18kDa band observed in Human Duodenum, Heart and Skeletal Muscle lysates and in Mouse and Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB) of anti-CFL2 antibody
(0. 003ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (WB) of anti-CFL2 antibody
(0. 001ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (WB) of anti-CFL2 antibody
HEK293 overexpressing Human Muscle Cofilin 2 (RC222215) and probed (mock transfection in first lane), tested by Origene.
NCBI/Uniprot data below describe general gene information for CFL2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_068733.1
[Other Products]
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_619579.1[Other Products]
NCBI GenBank Nucleotide #
NM_021914.7
[Other Products]
UniProt Secondary Accession #
G3V5P4[Other Products]
UniProt Related Accession #
Q9Y281[Other Products]
NCBI Official Full Name
cofilin-2 isoform 1
NCBI Official Synonym Full Names
cofilin 2
NCBI Official Symbol
CFL2??[Similar Products]
NCBI Official Synonym Symbols
NEM7
??[Similar Products]
NCBI Protein Information
cofilin-2
UniProt Protein Name
Cofilin-2
UniProt Synonym Protein Names
Cofilin, muscle isoform
UniProt Gene Name
CFL2??[Similar Products]
UniProt Entry Name
COF2_HUMAN
NCBI Summary for CFL2
This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Comments for CFL2
Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: actin cytoskeleton; extracellular space; I band; nuclear matrix
Molecular Function: actin binding; protein binding
Biological Process: actin filament depolymerization; muscle maintenance; positive regulation of actin filament depolymerization; sarcomere organization
Disease: Nemaline Myopathy 7
Research Articles on CFL2
1. The serum lever of Alzheimer's disease were increase and the expression of clf2 strongly correlated with the Mini-Mental State Examination scores of the AD patients
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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