CFL 2; CFL2; CFL-2; Cofilin 2 muscle; Cofilin; Cofilin muscle; Cofilin2; Cofilin 2; Cofilin-2; NEM 7; NEM7; Q9Y281; Cofilin-2; cofilin 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit IgG polyclonal antibody for Cofilin-2 (CFL2) detection.
Background: Cofilin 2 (muscle), also known as CFL2, is a protein which in humans is encoded by the CFL2 gene. It is mapped to 14q12. This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. And this protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western blot analysis of Cofilin 2 expression in rat liver extract (lane 1), mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). Cofilin 24 at 19KD was detected using rabbit anti- Cofilin 2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

Cofilin 2 was detected in paraffin-embedded sections of mouse cardiac muscle tissues using rabbit anti- Cofilin 2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

Cofilin 2 was detected in paraffin-embedded sections of rat skeletal muscle tissues using rabbit anti- Cofilin 2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

Cofilin 2 was detected in paraffin-embedded sections of human prostatic cancer tissues using rabbit anti- Cofilin 2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

– Da

cofilin 2

cofilin-2

Cofilin-2

COF2_HUMAN

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: extracellular space; I band

Molecular Function: protein binding

Biological Process: positive regulation of actin filament depolymerization

Disease: Nemaline Myopathy 7

1. "Entrez Gene: CFL2 cofilin 2 (muscle)".
2. Agrawal PB, Greenleaf RS, Tomczak KK, Lehtokari VL, Wallgren-Pettersson C, Wallefeld W, Laing NG, Darras BT, Maciver SK, Dormitzer PR, Beggs AH (January 2007). "Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2". Am. J. Hum. Genet. 80 (1): 162-7.
3. Gillett GT, Fox MF, Rowe PS, Casimir CM, Povey S (May 1996). "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum. Genet. 60 (Pt 3): 201-11.

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