Product Name
ARHGEF9, Polyclonal Antibody
Full Product Name
Anti-ARHGEF9 Antibody
Product Gene Name
anti-ARHGEF9 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human. Predicted: Mouse, Rat
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal ARHGEF9 (1) antibody was raised against a recombinated human ARHGEF9 protein 66-255aa (BC117406).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-ARHGEF9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARHGEF9 antibody
This gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
Applications Tested/Suitable for anti-ARHGEF9 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ARHGEF9 antibody
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
Testing Data of anti-ARHGEF9 antibody
NCBI/Uniprot data below describe general gene information for ARHGEF9. It may not necessarily be applicable to this product.
NCBI Accession #
BC117406
[Other Products]
UniProt Secondary Accession #
Q5JSL6; A8K1S8; B4DHC7; F8W7P8[Other Products]
UniProt Related Accession #
O43307[Other Products]
Molecular Weight
49,458 Da
NCBI Official Full Name
Homo sapiens Cdc42 guanine nucleotide exchange factor (GEF) 9, mRNA
NCBI Official Synonym Full Names
Cdc42 guanine nucleotide exchange factor (GEF) 9
NCBI Official Symbol
ARHGEF9??[Similar Products]
NCBI Official Synonym Symbols
PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN
??[Similar Products]
NCBI Protein Information
rho guanine nucleotide exchange factor 9; PEM-2 homolog; hPEM-2 collybistin; rac/Cdc42 guanine nucleotide exchange factor 9
UniProt Protein Name
Rho guanine nucleotide exchange factor 9
UniProt Synonym Protein Names
Collybistin; PEM-2 homolog; Rac/Cdc42 guanine nucleotide exchange factor 9
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
ARHGEF9??[Similar Products]
UniProt Entry Name
ARHG9_HUMAN
NCBI Summary for ARHGEF9
The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
UniProt Comments for ARHGEF9
ARHGEF9: Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters. Defects in ARHGEF9 are the cause of pileptic encephalopathy, early infantile, type 8 (EIEE8). A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: Xq11.1
Cellular Component: cytoplasm; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity
Biological Process: synaptic transmission; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; transmembrane transport
Disease: Epileptic Encephalopathy, Early Infantile, 8
Product References and Citations for anti-ARHGEF9 antibody
(1) Nagae,R., Sato,K., Yasui,Y., et al. Gs and Gq signalings regulate hPEM-2-induced cell responses in Neuro-2a cells. Biochem. Biophys. Res. Commun. 415 (1), 168-173 (2011).
Research Articles on ARHGEF9
1. The enhancement of Cb-induced gephyrin clustering by GTP-TC10 does not depend on the guanine nucleotide exchange activity of Cb but involves an interaction that resembles reported interactions of other small GTPases with their effectors
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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