Full Product Name
Anti-MEIS1 Monoclonal Antibody
Product Gene Name
anti-MEIS1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O00470
Species Reactivity
Human, Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human MEIS1 MEIS proteins belong to the TALE (Three Amino Acid Loop Extension) homeobox containing transcription factor family. MEIS1 has been associated with leukemogenesis and neuroblastoma.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-MEIS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MEIS1 antibody
Rabbit IgG Monoclonal antibody for O00470 detection. Tested positive for WB IP FC in Human Mouse Rat
Applications Tested/Suitable for anti-MEIS1 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-MEIS1 antibody
WB: 1:1000-1:5000
IP: 1:50
FC/FACS: 1:50
Western Blot (WB) of anti-MEIS1 antibody
Figure 1. Western blot analysis of MEIS1 using anti-MEIS1 antibody (M01602).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIS1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MEIS1.
NCBI/Uniprot data below describe general gene information for MEIS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002389.1
[Other Products]
NCBI GenBank Nucleotide #
NM_002398.3
[Other Products]
UniProt Primary Accession #
O00470
[Other Products]
UniProt Secondary Accession #
A8MV50[Other Products]
UniProt Related Accession #
O00470[Other Products]
Molecular Weight
50,576 Da
NCBI Official Full Name
homeobox protein Meis1
NCBI Official Synonym Full Names
Meis homeobox 1
NCBI Official Symbol
MEIS1??[Similar Products]
NCBI Protein Information
homeobox protein Meis1
UniProt Protein Name
Homeobox protein Meis1
Protein Family
Homeobox protein
UniProt Gene Name
MEIS1??[Similar Products]
NCBI Summary for MEIS1
Homeobox genes, of which the most well-characterized category is represented by the HOX genes, play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. [provided by RefSeq, Jul 2008]
UniProt Comments for MEIS1
Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
Research Articles on MEIS1
1. Our results showed that MEIS1 may have a negative role in regulation of MAML1expression during the esophageal squamous cell carcinoma progression.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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