Product Name
CNTNAP2, siRNA
Full Product Name
CNTNAP2 siRNA (Mouse)
Product Synonym Names
KIAA0868; Contactin-associated protein-like 2; Cell recognition molecule Caspr2
Product Gene Name
CNTNAP2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9CPW0
Specificity
CNTNAP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CNTNAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CNTNAP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CNTNAP2 sirna
siRNA to inhibit CNTNAP2 expression using RNA interference
Applications Tested/Suitable for CNTNAP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CNTNAP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001004357.2
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NCBI GenBank Nucleotide #
NM_001004357.2
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UniProt Primary Accession #
Q9CPW0
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UniProt Secondary Accession #
Q6P2K4; Q6ZQ31[Other Products]
UniProt Related Accession #
Q9CPW0[Other Products]
Molecular Weight
11,899 Da
NCBI Official Full Name
contactin-associated protein-like 2 isoform a
NCBI Official Synonym Full Names
contactin associated protein-like 2
NCBI Official Symbol
Cntnap2??[Similar Products]
NCBI Official Synonym Symbols
Caspr2; mKIAA0868; 5430425M22Rik
??[Similar Products]
NCBI Protein Information
contactin-associated protein-like 2
UniProt Protein Name
Contactin-associated protein-like 2
UniProt Synonym Protein Names
Cell recognition molecule Caspr2
Protein Family
Contactin-associated protein
UniProt Gene Name
Cntnap2??[Similar Products]
UniProt Synonym Gene Names
Kiaa0868??[Similar Products]
UniProt Entry Name
CNTP2_MOUSE
UniProt Comments for CNTNAP2
CNTNAP2: May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Associates with KCNA2. Predominantly expressed in nervous system. Belongs to the neurexin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; cell surface; cell projection; membrane; early endosome; integral to membrane; axolemma
Molecular Function: protein binding; enzyme binding; protease binding
Biological Process: ***** behavior; clustering of voltage-gated potassium channels; neuron recognition; social behavior; learning; cell adhesion; neurite development
Research Articles on CNTNAP2
1. Interaction proteomics revealed the interactors of Caspr2, including CNTN2, KCNAs, members of the ADAM family (ADAM22, ADAM23 and ADAM11), members of LGI family and MAGUKs (DLGs and MPPs).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
