Shank postsynaptic density protein; SH3 and multiple ankyrin repeat domains 3; Proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; PSAP2;; SH3 and multiple ankyrin repeat domains protein 3

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

S367-51

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Shank3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects a 190 kDa protein. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

Scientific Background: SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of ***** and developing brain to ensure normal development.

Neuroscience

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

1:1000 WB

Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.

122,694 Da[Similar Products]

SH3 and multiple ankyrin repeat domains 3

SH3 and multiple ankyrin repeat domains protein 3; SPANK-2; SH3/ankyrin domain gene 3; Shank postsynaptic density protein 3a; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2

SH3 and multiple ankyrin repeat domains protein 3

Prosap2; Shank3; ProSAP2??[Similar Products]

SHAN3_RAT

may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

SHANK3: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B. Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome). Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15). SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; neuron projection; cell; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; synapse; intracellular; cell junction

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; identical protein binding; protein binding; protein self-association; zinc ion binding; GKAP/Homer scaffold activity; actin binding; SH3 domain binding

Biological Process: negative regulation of actin filament bundle formation; MAPKKK cascade; learning; vocal learning; social behavior; positive regulation of synaptic transmission, glutamatergic; positive regulation of long-term neuronal synaptic plasticity; protein oligomerization; memory; positive regulation of synapse structural plasticity; synaptogenesis; striatal medium spiny neuron differentiation; ***** behavior; embryonic epithelial tube formation; brain morphogenesis; neuromuscular process controlling balance; negative regulation of cell volume

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