For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rabbit ADCY5. No significant cross-reactivity or interference between Rabbit ADCY5 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of ADCY5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280615 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenylate cyclase type 5 (ADCY5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADCY5. The ELISA analytical biochemical technique of the MBS280615 kit is based on ADCY5 antibody-ADCY5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADCY5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADCY5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ADCY5 in samples. An antibody specific for ADCY5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

139,624 Da

adenylate cyclase type 5

Adenylate cyclase type 5

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Mediates signaling downstream of ADRB1. Regulates the increase of free cytosolic Ca2+ in response to increased blood glucose levels and contributes to the regulation of Ca2+-dependent insulin secretion.

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