For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

Mouse

This antibody recognizes human, mouse, and rat SHANK1/SHANK3

100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

This product is stable for at least one (1) year at-20 degree C.

Small volumes of anti-SHANK1/SHANK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of ***** and developing brain to ensure normal development.

Immunofluorescence (IF)

IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system.

Detection ofSHANK1/SHANK3 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) SHANK1/SHANK3 stain.

185,505 Da

SH3 and multiple ankyrin repeat domains 3

SH3 and multiple ankyrin repeat domains protein 3

SH3 and multiple ankyrin repeat domains protein 3

Prosap2; Shank3; ProSAP2??[Similar Products]

may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

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