Product Name
BRAF35, Polyclonal Antibody
Full Product Name
BRAF35 antibody
Product Synonym Names
Polyclonal BRAF35; Anti-BRAF35; BRAF 35; BRAF -35; BRAF35; Breast Cancer Type 2 susceptibility protein-Associated Factor 35; BRCA2-Associated Factor 35
Product Gene Name
anti-BRAF35 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51587
Purity/Purification
BRAF35 antibody was purified by affinity chromatography.
Form/Format
Affinity purified IgG supplied in PBS with 0.02% NaN3.
Concentration
1.0 mg/ml (lot specific)
Biological Significance
Mutations of the human BRCA2 gene confer susceptibility to breast cancer. A structural DNA binding component has been identified and is designated as BRCA2-Associated Factor 35 (BRAF35). There is a close association of the BRAF35/BRCA2 complex with condensed chromatin coincident with histone H3 phosphorylation. Experimental evidence suggests a role for the BRCA2/BRAF35 complex in the modulation of cell cycle progression.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
BRAF35 antibody was raised in rabbit using residues 31-50 [KQERGEGPRA GEKGSHEEEP] of the human structural DNA-binding protein BRAF35 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-BRAF35 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BRAF35 antibody
Affinity purified Rabbit polyclonal BRAF35 antibody
Product Categories/Family for anti-BRAF35 antibody
Cancer; Purified Polyclonal Antibodies
Applications Tested/Suitable for anti-BRAF35 antibody
Western Blot (WB)
Application Notes for anti-BRAF35 antibody
WB: 1:500-1:1,000
NCBI/Uniprot data below describe general gene information for BRAF35. It may not necessarily be applicable to this product.
NCBI Accession #
AAB07223.1
[Other Products]
UniProt Primary Accession #
P51587
[Other Products]
UniProt Secondary Accession #
O00183; O15008; Q13879; Q5TBJ7[Other Products]
UniProt Related Accession #
P51587[Other Products]
Molecular Weight
384,225 Da
NCBI Official Full Name
BRCA2
NCBI Official Synonym Full Names
breast cancer 2, early onset
NCBI Official Symbol
BRCA2??[Similar Products]
NCBI Official Synonym Symbols
FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
??[Similar Products]
NCBI Protein Information
breast cancer type 2 susceptibility protein
UniProt Protein Name
Breast cancer type 2 susceptibility protein
UniProt Synonym Protein Names
Fanconi anemia group D1 protein
UniProt Gene Name
BRCA2??[Similar Products]
UniProt Synonym Gene Names
FACD; FANCD1??[Similar Products]
UniProt Entry Name
BRCA2_HUMAN
NCBI Summary for BRAF35
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
UniProt Comments for BRAF35
BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.
Protein type: DNA repair, damage; Nuclear receptor co-regulator; Tumor suppressor
Chromosomal Location of Human Ortholog: 13q12.3
Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule
Molecular Function: H4 histone acetyltransferase activity; gamma-tubulin binding; protein binding; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding
Biological Process: positive regulation of transcription, DNA-dependent; cell aging; cytokinesis; positive regulation of mitotic cell cycle; DNA repair; inner cell mass cell proliferation; oocyte maturation; regulation of cytokinesis; response to UV-C; negative regulation of mammary gland epithelial cell proliferation; double-strand break repair via homologous recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; hemopoiesis; replication fork protection; spermatogenesis; brain development; centrosome duplication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; female gonad development; response to X-ray
Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Breast Cancer; Medulloblastoma; Prostate Cancer; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1
Research Articles on BRAF35
1. BRCA2 tumors were more likely to be ER and HER2 positive than other non-BRCA familial breast cancers.
Precautions
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Disclaimer
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