Product Name
BRCA 2, Polyclonal Antibody
Full Product Name
BRCA 2 antibody
Product Synonym Names
Polyclonal BRCA 2; Anti-BRCA 2; BRCA-2; BRCA 2; BRCA -2; Breast cancer 2 early onset
Product Gene Name
anti-BRCA 2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse
Purity/Purification
BRCA 2 antibody was purified by affinity purification.
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Concentration
500 ug/ml (lot specific)
Biological Significance
BRCA 1 and 2 are breast cancer susceptibility proteins that play a role in DNA damage, repair, cell cycle progression, transcription and apoptosis, etc. The BRCA 1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs (double stranded breaks) in DNA. Cells lacking BRCA 1 and BRCA 2 cannot repair DSBs through the Rad51 dependent process.
Immunogen
BRCA 2 antibody was raised in Rabbit using synthetic peptide mapping to the N-terminus of human BRCA2 as the immunogen.
Preparation and Storage
Store at -20 degree C. For long-term storage, aliquot and freeze at -70 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-BRCA 2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BRCA 2 antibody
Rabbit polyclonal BRCA 2 antibody
Product Categories/Family for anti-BRCA 2 antibody
DNA & RNA
Applications Tested/Suitable for anti-BRCA 2 antibody
Western Blot (WB)
Application Notes for anti-BRCA 2 antibody
WB: 0.5-4 ug/ml
NCBI/Uniprot data below describe general gene information for BRCA 2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000050.2
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NCBI GenBank Nucleotide #
NM_000059.3
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UniProt Secondary Accession #
O00183; O15008; Q13879; Q5TBJ7[Other Products]
UniProt Related Accession #
P51587[Other Products]
Molecular Weight
384,225 Da
NCBI Official Full Name
breast cancer type 2 susceptibility protein
NCBI Official Synonym Full Names
breast cancer 2, early onset
NCBI Official Symbol
BRCA2??[Similar Products]
NCBI Official Synonym Symbols
FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
??[Similar Products]
NCBI Protein Information
breast cancer type 2 susceptibility protein
UniProt Protein Name
Breast cancer type 2 susceptibility protein
UniProt Synonym Protein Names
Fanconi anemia group D1 protein
UniProt Gene Name
BRCA2??[Similar Products]
UniProt Synonym Gene Names
FACD; FANCD1??[Similar Products]
UniProt Entry Name
BRCA2_HUMAN
NCBI Summary for BRCA 2
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
UniProt Comments for BRCA 2
BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.
Protein type: DNA repair, damage; Tumor suppressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 13q12.3
Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule
Molecular Function: H4 histone acetyltransferase activity; gamma-tubulin binding; protein binding; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding
Biological Process: positive regulation of transcription, DNA-dependent; cell aging; cytokinesis; DNA repair; positive regulation of mitotic cell cycle; inner cell mass cell proliferation; regulation of cytokinesis; oocyte maturation; response to UV-C; double-strand break repair via homologous recombination; negative regulation of mammary gland epithelial cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; spermatogenesis; hemopoiesis; replication fork protection; brain development; centrosome duplication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; female gonad development; response to X-ray
Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Breast Cancer; Medulloblastoma; Prostate Cancer; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1
Research Articles on BRCA 2
1. BRCA2 tumors were more likely to be ER and HER2 positive than other non-BRCA familial breast cancers.
Precautions
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Disclaimer
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