Full Product Name
BRCA2 Antibody
Product Synonym Names
Breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; BRCA2; FACD; FANCD1
Product Gene Name
anti-BRCA2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51587
Species Reactivity
Human, Rat
Specificity
BRCA2 Antibody detects endogenous levels of total BRCA2 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human BRCA2.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-BRCA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BRCA2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-BRCA2 antibody
IHC: 1:50~1:100
ELISA: 1:20000
Immunohistochemistry (IHC) of anti-BRCA2 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BRCA2 Antibody. The picture on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for BRCA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000050.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000059.3
[Other Products]
UniProt Primary Accession #
P51587
[Other Products]
UniProt Secondary Accession #
O00183; O15008; Q13879; Q5TBJ7[Other Products]
UniProt Related Accession #
P51587[Other Products]
NCBI Official Full Name
breast cancer type 2 susceptibility protein
NCBI Official Synonym Full Names
BRCA2, DNA repair associated
NCBI Official Symbol
BRCA2??[Similar Products]
NCBI Official Synonym Symbols
FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
??[Similar Products]
NCBI Protein Information
breast cancer type 2 susceptibility protein
UniProt Protein Name
Breast cancer type 2 susceptibility protein
UniProt Synonym Protein Names
Fanconi anemia group D1 protein
Protein Family
BRCA2 and CDKN1A-interacting protein
UniProt Gene Name
BRCA2??[Similar Products]
UniProt Synonym Gene Names
FACD; FANCD1??[Similar Products]
NCBI Summary for BRCA2
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
UniProt Comments for BRCA2
Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).
Research Articles on BRCA2
1. High BRCA2 expression is associated with drug resistance in ovarian cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
