For Research Use Only. Not for use in diagnostic procedures.

Store at 2 to 8 degree C for 6 months.

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Small volumes of 11betaHSD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1601285 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (11betaHSD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 11betaHSD2. The ELISA analytical biochemical technique of the MBS1601285 kit is based on 11betaHSD2 antibody-11betaHSD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 11betaHSD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 11betaHSD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (also known as 11?HSD2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse 11?HSD2 antibody. 11?HSD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse 11?HSD2 Antibody is added and binds to 11?HSD2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated 11?HSD2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse 11?HSD2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

44,127 Da

hydroxysteroid 11-beta dehydrogenase 2

corticosteroid 11-beta-dehydrogenase isozyme 2

Corticosteroid 11-beta-dehydrogenase isozyme 2

; 11-DH2; 11-beta-HSD2; 11-HSD type II; 11-beta-HSD type II; 11-beta-HSD??[Similar Products]

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

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