Product Name
HTR3A, siRNA
Full Product Name
HTR3A siRNA (Rat)
Product Synonym Names
5HT3; HTR3; 5-hydroxytryptamine receptor 3A; 5-HT3-A; 5-HT3A; 5-hydroxytryptamine receptor 3; 5-HT-3; 5-HT3R; Serotonin receptor 3A; Serotonin-gated ion channel receptor
Product Gene Name
HTR3A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35563
Specificity
HTR3A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HTR3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HTR3A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HTR3A sirna
siRNA to inhibit HTR3A expression using RNA interference
Applications Tested/Suitable for HTR3A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HTR3A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_077370.2
[Other Products]
NCBI GenBank Nucleotide #
NM_024394.2
[Other Products]
UniProt Primary Accession #
P35563
[Other Products]
UniProt Related Accession #
P35563[Other Products]
Molecular Weight
55,428 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 3A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 3A, ionotropic
NCBI Official Symbol
Htr3a??[Similar Products]
NCBI Official Synonym Symbols
5HT3; Htr3; 5-HT3; 5-HT3A
??[Similar Products]
NCBI Protein Information
5-hydroxytryptamine receptor 3A
UniProt Protein Name
5-hydroxytryptamine receptor 3A
UniProt Synonym Protein Names
5-hydroxytryptamine receptor 3; 5-HT-3; 5-HT3R; Serotonin receptor 3A; Serotonin-gated ion channel receptor
Protein Family
5-hydroxytryptamine receptor
UniProt Gene Name
Htr3a??[Similar Products]
UniProt Synonym Gene Names
5ht3; Htr3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R??[Similar Products]
UniProt Entry Name
5HT3A_RAT
NCBI Summary for HTR3A
receptor for 5-hydroxytryptamine (serotonin); functions as a ligand-gated ion channel [RGD, Feb 2006]
UniProt Comments for HTR3A
5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, cation; Channel, ligand-gated; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; axon; cytoplasm; plasma membrane; cell junction
Molecular Function: voltage-gated potassium channel activity; serotonin-activated cation-selective channel activity; serotonin binding; serotonin receptor activity
Biological Process: synaptic transmission; response to ethanol; response to cocaine; cation transport; serotonin receptor signaling pathway
Research Articles on HTR3A
1. The inhibition of I(5-HT3) by WIN55,212-2 is probably new one of peripheral analgesic mechanisms of WIN55,212-2, but the mechanism by which WIN55,212-2 inhibits I(5-HT3) warrants further investigation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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