Full Product Name
MSH6 Antibody
Product Gene Name
anti-MSH6 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9VUM0
Buffer
Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
IHC Positive Control
Colon, Colon Carcinoma
IHC Suggested Protocol
1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block ? Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab ? Dilute antibodies 1:100 ? 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Other Notes
Small volumes of anti-MSH6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MSH6 antibody
MSH6 (MutS Homolog 6) is part of the mismatch-repair pathway, often associated with hereditary non-polyposis colorectal cancer. Mutations to this protein have shown to be correlated with the development of sporadic colorectal carcinoma. Anti-MSH6 antibodies can be optimized when paired along with other antibodies such as MSH2, MLH1, and PMS2.
Applications Tested/Suitable for anti-MSH6 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-MSH6 antibody
Dilution Range: 1:100 - 1:200
Immunohistochemistry (IHC) of anti-MSH6 antibody
IHC (paraffin) of human esophagus using MSH6 Antibody
NCBI/Uniprot data below describe general gene information for MSH6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_648755.1
[Other Products]
NCBI GenBank Nucleotide #
NM_140498.2
[Other Products]
UniProt Primary Accession #
Q9VUM0
[Other Products]
UniProt Secondary Accession #
B6IDV3[Other Products]
UniProt Related Accession #
Q9VUM0[Other Products]
Molecular Weight
133,136 Da
NCBI Official Full Name
Msh6
NCBI Official Symbol
Msh6??[Similar Products]
NCBI Official Synonym Symbols
CG7003; DmelCG7003
??[Similar Products]
NCBI Protein Information
Msh6
UniProt Protein Name
Probable DNA mismatch repair protein Msh6
Protein Family
DNA mismatch repair protein
UniProt Gene Name
Msh6??[Similar Products]
UniProt Comments for MSH6
Involved in post-replicative DNA-mismatch repair.
Research Articles on MSH6
1. Heteroduplex DNA often goes unrepaired during meiotic recombination in an Msh6 mutant, leading to high levels of postmeiotic segregation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
