G/T mismatch binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; MutS alpha 160 kDa subunit; mutS homolog 6 (E Coli); p160

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

Mouse

Protein A purification

1mg/mL (lot specific)

Store at -20 degree C. Avoid freeze/ thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-MSH6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Cancer; Epigenetics

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Western Blot: 1:500-1:5000
Immunohistochemistry:1:50-1:500
Immunofluoresence: 1:50-1:500

Western Blot analysis of HEK293 cells using MSH6 Monoclonal Antibody at dilution of 1:1000

119,796 Da

mutS homolog 6

DNA mismatch repair protein Msh6

DNA mismatch repair protein Msh6

GTBP; hMSH6; GTBP; GTMBP; p160??[Similar Products]

MSH6_HUMAN

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]

MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; MutSalpha complex; nuclear chromosome; nucleoplasm; plasma membrane

Molecular Function: ADP binding; ATP binding; ATPase activity; double-stranded DNA binding; four-way junction DNA binding; guanine/thymine mispair binding; magnesium ion binding; methylated histone residue binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein binding; protein homodimerization activity; single guanine insertion binding; single thymine insertion binding

Biological Process: determination of ***** life span; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; isotype switching; maintenance of DNA repeat elements; meiotic mismatch repair; meiotic recombination; mismatch repair; negative regulation of DNA recombination; positive regulation of helicase activity; response to UV; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome

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