For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against MUSK. It has been selected for its ability to recognize MUSK in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-MUSK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Human Jurkat Cells.

Western Blot: Sample: Recombinant MUSK, Mouse.

DAB staining on IHC-P; Samples: Mouse Liver Tissue

DAB staining on IHC-P; Samples: Mouse Heart Tissue.

98,081 Da

muscle, skeletal, receptor tyrosine kinase

muscle, skeletal receptor tyrosine-protein kinase

Muscle, skeletal receptor tyrosine-protein kinase

Nsk2; MuSK; Muscle-specific kinase receptor??[Similar Products]

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Musk family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 4 B3|4 31.87 cM

Cellular Component: cell junction; cell projection; external side of plasma membrane; integral component of membrane; integral component of plasma membrane; membrane; neuromuscular junction; plasma membrane; postsynaptic membrane; receptor complex; synapse

Molecular Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; PDZ domain binding; protein binding; protein kinase activity; protein kinase binding; protein tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell differentiation; memory; multicellular organism development; negative regulation of gene expression; neuromuscular junction development; phosphorylation; positive regulation of gene expression; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein geranylgeranylation; positive regulation of protein phosphorylation; positive regulation of skeletal muscle acetylcholine-gated channel clustering; positive regulation of synaptic growth at neuromuscular junction; positive regulation of synaptic transmission, cholinergic; protein amino acid phosphorylation; protein autophosphorylation; receptor clustering; regulation of synaptic growth at neuromuscular junction; regulation of transcription, DNA-templated; response to electrical stimulus; skeletal muscle acetylcholine-gated channel clustering

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