Product Name
ATP6V0A2, siRNA
Full Product Name
ATP6V0A2 siRNA (Mouse)
Product Synonym Names
ATP6N1B; TJ6; V-type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; Immune suppressor factor J6B7; ISF; Lysosomal H(+)-transporting ATPase V0 subunit a2; ShIF; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Product Gene Name
ATP6V0A2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15920
Specificity
ATP6V0A2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP6V0A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP6V0A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP6V0A2 sirna
siRNA to inhibit ATP6V0A2 expression using RNA interference
Applications Tested/Suitable for ATP6V0A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP6V0A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035726.2
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NCBI GenBank Nucleotide #
NM_011596.5
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UniProt Primary Accession #
P15920
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UniProt Secondary Accession #
Q3U2X3; Q8VHU0; Q9JHJ2; A4FU82[Other Products]
UniProt Related Accession #
P15920[Other Products]
Molecular Weight
29,740 Da
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 2
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal V0 subunit A2
NCBI Official Symbol
Atp6v0a2??[Similar Products]
NCBI Official Synonym Symbols
ISF; Tj6; J6B7; SHIF; Stv1; TJ6M; TJ6s; ATP6a2; Atp6n2; C76904; Atp6n1d; AI385560; AW489264; V-ATPase a2; 8430408C20Rik; V-ATPase 116 kDa
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NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 2
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 2
UniProt Synonym Protein Names
Immune suppressor factor J6B7; ISF; Lysosomal H(+)-transporting ATPase V0 subunit a2; ShIF; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Protein Family
V-type proton ATPase
UniProt Gene Name
Atp6v0a2??[Similar Products]
UniProt Synonym Gene Names
Atp6n1b; Tj6; V-ATPase 116 kDa isoform a2; ISF??[Similar Products]
UniProt Entry Name
VPP2_MOUSE
NCBI Summary for ATP6V0A2
This gene encodes a subunit of vacuolar ATPase, a multimeric enzyme that localizes to intracellular vesicles and to the plasma membrane of specialized cells. The encoded protein is a component of the V(0) domain, which functions in proton translocation across membranes. Function of this gene is important in fetal-specific immune suppression during pregnancy. [provided by RefSeq, May 2013]
UniProt Comments for ATP6V0A2
ATP6V0A2: Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. Defects in ATP6V0A2 are the cause of cutis laxa autosomal recessive type 2A (ARCL2A). An autosomal recessive disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, a general connective tissue weakness, and varying degrees of growth and developmental delay and neurological abnormalities. Some affected individuals develop seizures and mental deterioration later in life, whereas the skin phenotype tends to become milder with age. At the molecular level, an abnormal glycosylation of serum proteins is observed in many cases. Defects in ATP6V0A2 are a cause of wrinkly skin syndrome (WSS). WSS is rare autosomal recessive disorder characterized by wrinkling of the skin of the dorsum of the hands and feet, an increased number of palmar and plantar creases, wrinkled abdominal skin, multiple musculoskeletal abnormalities, microcephaly, growth failure and developmental delay. Belongs to the V-ATPase 116 kDa subunit family.
Protein type: Transporter, ion channel; Membrane protein, multi-pass; Transporter; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter, iron
Cellular Component: focal adhesion; membrane; lysosomal membrane; cytoplasm; integral to membrane; acrosome; plasma membrane; vacuolar proton-transporting V-type ATPase complex; endosome
Molecular Function: protein binding; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding
Biological Process: vacuolar acidification; proton transport; ATP synthesis coupled proton transport; transport; ATP hydrolysis coupled proton transport; ion transport
Research Articles on ATP6V0A2
1. These observations suggest that a2V plays a crucial role in spermatogenesis by regulating testicular immune responses, and its inhibition in males leads to poor pregnancy outcome in females.
Precautions
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Disclaimer
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