DADB-122G4.1; CO2; DKFZp779M0311; C3/C5 convertase; complement component C2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human C2. No significant cross-reactivity or interference between Human C2 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of C2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2800207 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement C2 (C2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C2. The ELISA analytical biochemical technique of the MBS2800207 kit is based on C2 antibody-C2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs a two-site sandwich ELISA to quantitate C2 in samples. An antibody specific for C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

69,444 Da

complement C2

complement C2

Complement C2

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.

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