Product Name
Keratin K74 (KRT74), Polyclonal Antibody
Full Product Name
Keratin K74 antibody
Product Synonym Names
Polyclonal Keratin K74; Anti Keratin K74; K5C; Keratin K-74; KRT5C; Keratin K74; KB37; CK-74; Keratin K 74; Cytokeratin 74; K74
Product Gene Name
anti-KRT74 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7RTS7
Specificity
Human Keratin K74
Form/Format
Supplied in liquid form. Contains 0.09% sodium azide
Biological Significance
Keratin 73 is a keratin protein. Keratin refers to a family of fibrous structural proteins. Keratin is the key structural material making up the outer layer of human skin. It is also the key structural component of hair and nails. Keratin monomers assemble into bundles to form intermediate filaments, which are tough and insoluble and form strong unmineralized tissues found in reptiles, birds, amphibians, and mammals. The only other
biological matter known to approximate the toughness of keratinized tissue is chitin.
Immunogen
Keratin K74 antibody was raised in Guinea Pig using synthetic peptide of human keratin K74 coupled to KLH as the immunogen.
Preparation and Storage
Store at 2-8 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-KRT74 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT74 antibody
Guinea Pig polyclonal Keratin K74 antibody
Product Categories/Family for anti-KRT74 antibody
Cell Biology
Applications Tested/Suitable for anti-KRT74 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-KRT74 antibody
IHC: 1:100-200
WB: 1:2000
NCBI/Uniprot data below describe general gene information for KRT74. It may not necessarily be applicable to this product.
NCBI Accession #
NP_778223.2
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NCBI GenBank Nucleotide #
NM_175053.3
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UniProt Primary Accession #
Q7RTS7
[Other Products]
UniProt Secondary Accession #
Q86Y45; B5MD61[Other Products]
UniProt Related Accession #
Q7RTS7[Other Products]
Molecular Weight
57,865 Da[Similar Products]
NCBI Official Full Name
keratin, type II cytoskeletal 74
NCBI Official Synonym Full Names
keratin 74, type II
NCBI Official Symbol
KRT74??[Similar Products]
NCBI Official Synonym Symbols
ADWH; HTSS2; HYPT3; KRT5C; K6IRS4; KRT6IRS4
??[Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 74
UniProt Protein Name
Keratin, type II cytoskeletal 74
UniProt Synonym Protein Names
Cytokeratin-74; CK-74; Keratin-5c; K5C; Keratin-74; K74; Type II inner root sheath-specific keratin-K6irs4; Type-II keratin Kb37
UniProt Gene Name
KRT74??[Similar Products]
UniProt Synonym Gene Names
K6IRS4; KB37; KRT5C; KRT6IRS4; CK-74; K5C; K74??[Similar Products]
UniProt Entry Name
K2C74_HUMAN
NCBI Summary for KRT74
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This protein belongs to a family of keratins that are specifically expressed in the inner root sheath of hair follicles.[provided by RefSeq, Jun 2009]
UniProt Comments for KRT74
KRT74: Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle (Probable). Defects in KRT74 are a cause of woolly hair autosomal dominant (ADWH). A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrhexis nodosa and tapered ends. Defects in KRT74 are the cause of hypotrichosis type 3 (HYPT3). A condition characterized by the presence of less than the normal amount of hair. Affected individuals have normal hair in early childhood but experience progressive hair loss limited to the scalp beginning in the middle of the first decade and almost complete baldness by the third decade. Body hair, beard, eyebrows, axillary hair, teeth, and nails develop normally. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cytoplasm; keratin filament
Molecular Function: structural molecule activity
Biological Process: intermediate filament cytoskeleton organization and biogenesis
Disease: Ectodermal Dysplasia 7, Hair/nail Type; Woolly Hair, Autosomal Dominant; Hypotrichosis 3
Research Articles on KRT74
1. These observations show for the first time that homozygosity for a KRT74 missense variant may be associated with autosomal recessive pure hair and nail ectodermal dysplasia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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