Product Name
BRCA2, siRNA
Full Product Name
BRCA2 siRNA (Mouse)
Product Synonym Names
FANCD1; Breast cancer type 2 susceptibility protein homolog; Fanconi anemia group D1 protein homolog
Product Gene Name
BRCA2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P97929
Specificity
BRCA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BRCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BRCA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BRCA2 sirna
siRNA to inhibit BRCA2 expression using RNA interference
Applications Tested/Suitable for BRCA2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BRCA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074470.1
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NCBI GenBank Nucleotide #
NM_001081001.2
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UniProt Primary Accession #
P97929
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UniProt Secondary Accession #
O35922; P97383; F8VPU5[Other Products]
UniProt Related Accession #
P97929[Other Products]
Molecular Weight
370,664 Da
NCBI Official Full Name
breast cancer type 2 susceptibility protein homolog
NCBI Official Synonym Full Names
breast cancer 2
NCBI Official Symbol
Brca2??[Similar Products]
NCBI Official Synonym Symbols
Fancd1; RAB163
??[Similar Products]
NCBI Protein Information
breast cancer type 2 susceptibility protein homolog
UniProt Protein Name
Breast cancer type 2 susceptibility protein homolog
UniProt Synonym Protein Names
Fanconi anemia group D1 protein homolog
Protein Family
BRCA2 and CDKN1A-interacting protein
UniProt Gene Name
Brca2??[Similar Products]
UniProt Synonym Gene Names
Fancd1??[Similar Products]
UniProt Entry Name
BRCA2_MOUSE
UniProt Comments for BRCA2
BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.
Protein type: DNA repair, damage; Nuclear receptor co-regulator; Tumor suppressor
Cellular Component: nucleoplasm; centrosome; protein complex; cytoskeleton; cytoplasm; BRCA2-MAGE-D1 complex; intracellular; nucleus; secretory granule
Molecular Function: protein binding; gamma-tubulin binding; H4 histone acetyltransferase activity; DNA binding; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding
Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; positive regulation of mitotic cell cycle; chromosome organization and biogenesis; homologous chromosome orientation during meiosis; DNA recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; mammary gland development; double-strand break repair; response to gamma radiation; hemopoiesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; female gonad development; response to X-ray; chordate embryonic development; multicellular organism growth; cell aging; cell cycle; DNA repair; regulation of cytokinesis; inner cell mass cell proliferation; oocyte maturation; response to UV-C; negative regulation of mammary gland epithelial cell proliferation; double-strand break repair via homologous recombination; regulation of cell proliferation; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; male meiosis I; nucleotide-excision repair; spermatogenesis; replication fork protection; brain development; response to DNA damage stimulus; centrosome duplication
Research Articles on BRCA2
1. BRCA2 exon 27 domain maintains chromosomal integrity at both stalled and collapsed replication forks consistent with involvement in both replication fork maintenance and double strand break repair.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
