Product Name
ARHGEF9, siRNA
Full Product Name
ARHGEF9 siRNA (Human)
Product Synonym Names
ARHDH9; KIAA0424; Rho guanine nucleotide exchange factor 9; Collybistin; PEM-2 homolog; Rac/Cdc42 guanine nucleotide exchange factor 9
Product Gene Name
ARHGEF9 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43307
Specificity
ARHGEF9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARHGEF9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARHGEF9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARHGEF9 sirna
siRNA to inhibit ARHGEF9 expression using RNA interference
Applications Tested/Suitable for ARHGEF9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARHGEF9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001166950.1
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NCBI GenBank Nucleotide #
NM_001173479.1
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UniProt Primary Accession #
O43307
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UniProt Secondary Accession #
Q5JSL6; A8K1S8; B4DHC7; F8W7P8[Other Products]
UniProt Related Accession #
O43307[Other Products]
Molecular Weight
49,458 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 9 isoform 2
NCBI Official Synonym Full Names
Cdc42 guanine nucleotide exchange factor (GEF) 9
NCBI Official Symbol
ARHGEF9??[Similar Products]
NCBI Official Synonym Symbols
PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN
??[Similar Products]
NCBI Protein Information
rho guanine nucleotide exchange factor 9
UniProt Protein Name
Rho guanine nucleotide exchange factor 9
UniProt Synonym Protein Names
Collybistin; PEM-2 homolog; Rac/Cdc42 guanine nucleotide exchange factor 9
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
ARHGEF9??[Similar Products]
UniProt Synonym Gene Names
ARHDH9; KIAA0424??[Similar Products]
UniProt Entry Name
ARHG9_HUMAN
NCBI Summary for ARHGEF9
The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
UniProt Comments for ARHGEF9
ARHGEF9: Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters. Defects in ARHGEF9 are the cause of pileptic encephalopathy, early infantile, type 8 (EIEE8). A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: Xq11.1
Cellular Component: cytoplasm; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity
Biological Process: synaptic transmission; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; transmembrane transport
Disease: Epileptic Encephalopathy, Early Infantile, 8
Research Articles on ARHGEF9
1. Impairment of the membrane lipid binding activity of Collybistin R290H and a consequent defect in inhibitory synapse maturation represent a likely molecular pathomechanism of epilepsy and mental retardation in humans.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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