For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C.

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Small volumes of HSD11b2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9137116 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD11b2. The ELISA analytical biochemical technique of the MBS9137116 kit is based on HSD11b2 antibody-HSD11b2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD11b2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD11b2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the sample, this Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

44,127 Da

hydroxysteroid 11-beta dehydrogenase 2

corticosteroid 11-beta-dehydrogenase isozyme 2

Corticosteroid 11-beta-dehydrogenase isozyme 2

; 11-DH2; 11-beta-HSD2; 11-HSD type II; 11-beta-HSD type II; 11-beta-HSD??[Similar Products]

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

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