Product Name
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ispd), Recombinant Protein
Full Product Name
Recombinant Danio rerio 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ispd)
Product Gene Name
ispd recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-462aa, full length protein
Sequence
MGTVTIQLSC LRHIRKLCFS CPWEGRRLFK MLLQFHHETQ RPLDPGCLLP QDAERSADQP GRAVVDFPVA AVLPAGGSGE RMGLTTPKQF CSIFNRPLIS YTIQAFERLP WIVMVVVVVA KDNHDLMLNI VRKFNHTKVK VVHGGTTRHR SIYNGLQAFS DSTDSSTPKP KVVIIHDAVR PFVEEDLLLK ITLAAKEQGA SGAIRPLVST VIATTSESYL DHSLERAKYR ASEMPQGFLY DIIFQAYQRC SEFDLEFGTE CLHLALQYCG TNARLIEGPP TLWKVTYKRD LAAAEAIIKE TLSVSACIIA EAEEEAVELA KTLQKNLNMM ETDVIPCGKE SNVQYLSKTR NFIHISASAS SSLWVLEMVK CFEDIDHARL YPVVIVWVQL SMTKQSADSQ ETDEFMALAS EVKQRNVLLY GIKIDHSKEL EQWQRSLERL GQITLVLIRD RNMALTGQML HV
3D Structure
ModBase 3D Structure for A0JPF9
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of ispd recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for ispd. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001071270.1
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NCBI GenBank Nucleotide #
NM_001077802.1
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UniProt Primary Accession #
A0JPF9
[Other Products]
UniProt Secondary Accession #
E9QIE3; F1R603[Other Products]
UniProt Related Accession #
A0JPF9[Other Products]
Molecular Weight
48,502 Da
NCBI Official Full Name
D-ribitol-5-phosphate cytidylyltransferase
NCBI Official Synonym Full Names
isoprenoid synthase domain containing
NCBI Official Symbol
ispd??[Similar Products]
NCBI Official Synonym Symbols
sb:eu371; zgc:154151
??[Similar Products]
NCBI Protein Information
D-ribitol-5-phosphate cytidylyltransferase; isoprenoid synthase domain-containing protein
UniProt Protein Name
D-ribitol-5-phosphate cytidylyltransferase
UniProt Synonym Protein Names
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein
Protein Family
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProt Gene Name
ispd??[Similar Products]
UniProt Comments for ispd
Cytidylyltransferase required for protein O-linked mannosylation (PubMed:22522421). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively. Not Involved in dolichol production.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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