Full Product Name
LIPA siRNA (Human)
Product Synonym Names
Lysosomal acid lipase/cholesteryl ester hydrolase; Acid cholesteryl ester hydrolase; LAL; Cholesteryl esterase; Lipase A; Sterol esterase
Product Gene Name
LIPA sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P38571
Specificity
LIPA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIPA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LIPA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LIPA sirna
siRNA to inhibit LIPA expression using RNA interference
Applications Tested/Suitable for LIPA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LIPA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000226.2
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NCBI GenBank Nucleotide #
NM_000235.3
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UniProt Primary Accession #
P38571
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UniProt Secondary Accession #
Q16529; Q53H21; Q5T074; Q5T771; Q96EJ0; B2RBH5; D3DR29[Other Products]
UniProt Related Accession #
P38571[Other Products]
Molecular Weight
39,112 Da
NCBI Official Full Name
lysosomal acid lipase/cholesteryl ester hydrolase isoform 1
NCBI Official Synonym Full Names
lipase A, lysosomal acid, cholesterol esterase
NCBI Official Symbol
LIPA??[Similar Products]
NCBI Official Synonym Symbols
LAL; CESD
??[Similar Products]
NCBI Protein Information
lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Protein Name
Lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Synonym Protein Names
Cholesteryl esterase; Lipase A; Sterol esterase
UniProt Gene Name
LIPA??[Similar Products]
UniProt Synonym Gene Names
Acid cholesteryl ester hydrolase; LAL??[Similar Products]
UniProt Entry Name
LICH_HUMAN
NCBI Summary for LIPA
This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]
UniProt Comments for LIPA
LIPA: Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor- mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. Defects in LIPA are the cause of Wolman disease (WOD). WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year. Defects in LIPA are the cause of cholesteryl ester storage disease (CESD). CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - steroid biosynthesis; Hydrolase; EC 3.1.1.13
Chromosomal Location of Human Ortholog: 10q23.2-q23.3
Cellular Component: lysosome
Molecular Function: sterol esterase activity; lipase activity
Biological Process: cell proliferation; homeostasis of number of cells within a tissue; tissue remodeling; cell morphogenesis; cytokine production; inflammatory response; lipid catabolic process; lung development
Disease: Lysosomal Acid Lipase Deficiency
Research Articles on LIPA
1. Wolmans disease is a rare autosomal recessive lysosomal storage disease.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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