Product Name
LIPA/Lysosomal Acid Lipase, Monoclonal Antibody
Full Product Name
Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9)
Product Synonym Names
Rabbit Polyclonal to Human LIPA/Lysosomal Acid Lipase; LIPA; Cholesterol ester hydrolase; Cholesteryl esterase; LAL; Lysosomal acid lipase; Lipase A; Sterol esterase; CESD
Product Gene Name
anti-LIPA antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P38571
Specificity
Human LIPA/Lysosomal Acid Lipase
Purity/Purification
Purified from ascites by Protein A
Immunogen
LIPA (AAH12287, aa1-399 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C or lower.
Aliquot to avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-LIPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LIPA antibody
intracellular hydrolysis of cholesteryl esters and triglycerides thathave been internalized via receptor-mediated endocytosis of lipoproteinparticles. Important in mediating the effect of LDL (low densitylipoprotein) uptake on suppression of hydroxymethylglutaryl-CoAreductase and activation of endogenous cellular cholesteryl esterformation.
Applications Tested/Suitable for anti-LIPA antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-LIPA antibody
IHC-P: 10 ug/ml
Immunohistochemistry (IHC) of anti-LIPA antibody
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot (WB) of anti-LIPA antibody
Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation (IP) of anti-LIPA antibody
Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.
ELISA (EIA) of anti-LIPA antibody
Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.
NCBI/Uniprot data below describe general gene information for LIPA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000226.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000235.3
[Other Products]
UniProt Primary Accession #
P38571
[Other Products]
UniProt Secondary Accession #
Q16529; Q53H21; Q5T074; Q5T771; Q96EJ0; B2RBH5; D3DR29[Other Products]
UniProt Related Accession #
P38571[Other Products]
Molecular Weight
39,112 Da
NCBI Official Full Name
lysosomal acid lipase/cholesteryl ester hydrolase isoform 1
NCBI Official Synonym Full Names
lipase A, lysosomal acid type
NCBI Official Symbol
LIPA??[Similar Products]
NCBI Official Synonym Symbols
LAL; CESD
??[Similar Products]
NCBI Protein Information
lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Protein Name
Lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Synonym Protein Names
Cholesteryl esterase; Lipase A; Sterol esterase
Protein Family
Lipoprotein
UniProt Gene Name
LIPA??[Similar Products]
UniProt Synonym Gene Names
Acid cholesteryl ester hydrolase; LAL??[Similar Products]
NCBI Summary for LIPA
This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]
UniProt Comments for LIPA
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.
Research Articles on LIPA
1. LAL activity is significantly reduced in NAFLD, compared to that in HCV patients. This finding is particularly evident in the pre-cirrhotic stage of disease. LAL activity is also correlated with platelet and white blood cell count, suggesting an analytic interference of portal-hypertension-induced pancytopenia on DBS-determined LAL activity.
Precautions
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Disclaimer
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