For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of AIF-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7606868 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AIF-1 (Allograft inflammatory factor 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AIF-1. The ELISA analytical biochemical technique of the MBS7606868 kit is based on AIF-1 antibody-AIF-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AIF-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AIF-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AIF-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AIF-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AIF-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AIF-1 can be calculated.

16,827 Da

allograft inflammatory factor 1

allograft inflammatory factor 1

Allograft inflammatory factor 1

Iba1; Mrf1; AIF-1??[Similar Products]

may play a role in macrophage activation and function [RGD, Feb 2006]

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: actin filament; cell projection; cytoplasm; cytosol; lamellipodium; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup; ruffle

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; actin filament polymerization; cellular response to extracellular stimulus; cellular response to hormone stimulus; inflammatory response; negative regulation of apoptosis; negative regulation of smooth muscle cell proliferation; phagocytosis, engulfment; positive regulation of cell migration; positive regulation of muscle hyperplasia; positive regulation of nitric oxide biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; Rac protein signal transduction; response to axon injury; response to cytokine stimulus; response to electrical stimulus; response to glucocorticoid stimulus

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