Product Name
ATP6V0A2, siRNA
Full Product Name
ATP6V0A2 siRNA (Human)
Product Synonym Names
V-type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; Lysosomal H(+)-transporting ATPase V0 subunit a2; TJ6; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Product Gene Name
ATP6V0A2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y487
Specificity
ATP6V0A2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP6V0A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP6V0A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP6V0A2 sirna
siRNA to inhibit ATP6V0A2 expression using RNA interference
Applications Tested/Suitable for ATP6V0A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP6V0A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036595.2
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NCBI GenBank Nucleotide #
NM_012463.3
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UniProt Primary Accession #
Q9Y487
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UniProt Secondary Accession #
Q6NUM0; A8K026[Other Products]
UniProt Related Accession #
Q9Y487[Other Products]
Molecular Weight
98,082 Da
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 2
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal V0 subunit a2
NCBI Official Symbol
ATP6V0A2??[Similar Products]
NCBI Official Synonym Symbols
A2; RTF; TJ6; WSS; ARCL; J6B7; STV1; TJ6M; TJ6S; VPH1; ARCL2A; ATP6A2; ATP6N1D
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NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 2
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 2
UniProt Synonym Protein Names
Lysosomal H(+)-transporting ATPase V0 subunit a2; TJ6; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Protein Family
V-type proton ATPase
UniProt Gene Name
ATP6V0A2??[Similar Products]
UniProt Synonym Gene Names
V-ATPase 116 kDa isoform a2??[Similar Products]
UniProt Entry Name
VPP2_HUMAN
NCBI Summary for ATP6V0A2
The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009]
UniProt Comments for ATP6V0A2
ATP6V0A2: Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. Defects in ATP6V0A2 are the cause of cutis laxa autosomal recessive type 2A (ARCL2A). An autosomal recessive disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, a general connective tissue weakness, and varying degrees of growth and developmental delay and neurological abnormalities. Some affected individuals develop seizures and mental deterioration later in life, whereas the skin phenotype tends to become milder with age. At the molecular level, an abnormal glycosylation of serum proteins is observed in many cases. Defects in ATP6V0A2 are a cause of wrinkly skin syndrome (WSS). WSS is rare autosomal recessive disorder characterized by wrinkling of the skin of the dorsum of the hands and feet, an increased number of palmar and plantar creases, wrinkled abdominal skin, multiple musculoskeletal abnormalities, microcephaly, growth failure and developmental delay. Belongs to the V-ATPase 116 kDa subunit family.
Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, iron; Transporter, ion channel; Energy Metabolism - oxidative phosphorylation
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: phagocytic vesicle membrane; focal adhesion; lysosomal membrane; cytoplasm; plasma membrane; integral to membrane; acrosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex
Molecular Function: protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding
Biological Process: interaction with host; vacuolar acidification; ATP synthesis coupled proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; immune response; transmembrane transport
Disease: Cutis Laxa, Autosomal Recessive, Type Iia; Wrinkly Skin Syndrome
Research Articles on ATP6V0A2
1. The granule-associated a2V isoform has a role in maintaining a pH gradient within the cell between the cytosol and granules in neutrophils.
Precautions
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