Full Product Name
Anti-Human CD49d Monoclonal Antibody (Biotin Conjugated)
Product Synonym Names
Human CD49d; Integrin alpha-4; ITGA4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD49d
Product Gene Name
anti-CD49d antibody
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Matching Pairs
Antibody: CD49d Clone #BU49 (MBS2553814)
Isotype Control: IgG1, kappa Clone #MOPC-21 (MBS2557745)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P13612
Form/Format
Liquid; PBS with 0.05% Proclin300, 1% BSA
Cellular Localization
Membrane
Preparation and Storage
Store at 2-8 degree C. Protected from prolonged exposure to light.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CD49d antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD49d antibody
CD49d is a 150 kD alpha integrin chain known as alpha4 integrin or VLA-4 alpha chain. It forms a heterodimer with either integrin beta1 (alpha4beta1, VLA-4) or beta7 (alpha4beta7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. alpha4beta7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.
Applications Tested/Suitable for anti-CD49d antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD49d antibody
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <=1.0 ug per 10e6 cells in 100 ul volume or 100 ul of whole blood.
NCBI/Uniprot data below describe general gene information for CD49d. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000876.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000885.6
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UniProt Primary Accession #
P13612
[Other Products]
UniProt Secondary Accession #
Q7Z4L6; D3DPG4[Other Products]
UniProt Related Accession #
P13612[Other Products]
Molecular Weight
21,536 Da
NCBI Official Full Name
integrin alpha-4 isoform 1 preproprotein
NCBI Official Synonym Full Names
integrin subunit alpha 4
NCBI Official Symbol
ITGA4??[Similar Products]
NCBI Official Synonym Symbols
IA4; CD49D
??[Similar Products]
NCBI Protein Information
integrin alpha-4
UniProt Protein Name
Integrin alpha-4
UniProt Synonym Protein Names
CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD_antigen: CD49d
UniProt Gene Name
ITGA4??[Similar Products]
UniProt Synonym Gene Names
CD49D??[Similar Products]
NCBI Summary for CD49d
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Comments for CD49d
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand- (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
Product References and Citations for anti-CD49d antibody
1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.
Research Articles on CD49d
1. study suggests a novel association of ITGA4 +3061A/G polymorphism with AD and its possible contribution to the disease pathology.
Precautions
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Disclaimer
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