DR6

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

Luke-1

Rat: Purified from concentrated hybridoma tissue culture supernatant.

Recognizes human DR6.

Liquid. In PBS containing 0.02% sodium azide.

Long term: -20 degree C
Short term: 4 degree C
Avoid freeze/thaw cycles. Stable for at least 1 year after receipt when stored as recommended.

Small volumes of anti-Death receptor-6; TNFRSF 21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Apoptosis is induced by certain cytokines including TNF and Fas ligand (CD95L) of the TNF family through their death domain containing receptors, TNF-R1 and Fas (CD95). Several death receptors including DR3, TRAIL-R1, and TRAIL-R2 were identified. A death domain containing receptor in the TNF-R family was cloned and termed DR6 for death receptor-6. Like TNF-R1, DR6 interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kappaB and JNK. DR6 is widely expressed in human tissues and cell lines. The ligand for DR6 has not been identified yet. T cells from DR6-deficient mice showed preference toward Th2 differentiation in vitro, suggesting that DR6, working through JNK functions to attenuate the Th2 response. Other studies of DR6-targeted mutant mice demonstrated that DR6 functions as a regulatory receptor for mediating CD4(+) T cell activation and maintaining proper immune responses.

Cell Signaling

Flow Cytometry (FC/FACS)

Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line).Method: Cells (5x105) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1ug/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.

71,845 Da

tumor necrosis factor receptor superfamily member 21

tumor necrosis factor receptor superfamily member 21

Tumor necrosis factor receptor superfamily member 21

DR6??[Similar Products]

TNR21_HUMAN

This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation. [provided by RefSeq, Jul 2013]

DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in ***** liver and peripheral blood leukocytes.

Protein type: Membrane protein, integral; Receptor, cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: axon; integral to plasma membrane; intrinsic to plasma membrane; plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity

Biological Process: adaptive immune response; apoptosis; B cell apoptosis; cellular lipid metabolic process; humoral immune response; immune response; inflammatory response; myelination; negative regulation of B cell proliferation; negative regulation of myelination; negative regulation of T cell proliferation; neuron apoptosis; regulation of apoptosis; regulation of cell proliferation; regulation of oligodendrocyte differentiation; response to lipopolysaccharide; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.