Product Name
ESCO2, siRNA
Full Product Name
ESCO2 siRNA (Mouse)
Product Synonym Names
N-acetyltransferase ESCO2; Establishment of cohesion 1 homolog 2; ECO1 homolog 2
Product Gene Name
ESCO2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CIB9
Specificity
ESCO2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ESCO2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ESCO2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ESCO2 sirna
siRNA to inhibit ESCO2 expression using RNA interference
Applications Tested/Suitable for ESCO2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ESCO2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_082315.3
[Other Products]
NCBI GenBank Nucleotide #
NM_028039.2
[Other Products]
UniProt Primary Accession #
Q8CIB9
[Other Products]
UniProt Secondary Accession #
Q3UMT6; Q6IQX5; Q8BNG9; Q8BQF8; Q9CRI8[Other Products]
UniProt Related Accession #
Q8CIB9[Other Products]
Molecular Weight
67,273 Da
NCBI Official Full Name
N-acetyltransferase ESCO2
NCBI Official Synonym Full Names
establishment of cohesion 1 homolog 2 (S. cerevisiae)
NCBI Official Symbol
Esco2??[Similar Products]
NCBI Official Synonym Symbols
2410004I17Rik; D030072L07Rik
??[Similar Products]
NCBI Protein Information
N-acetyltransferase ESCO2
UniProt Protein Name
N-acetyltransferase ESCO2
UniProt Synonym Protein Names
Establishment of cohesion 1 homolog 2; ECO1 homolog 2
Protein Family
N-acetyltransferase
UniProt Gene Name
Esco2??[Similar Products]
UniProt Synonym Gene Names
ECO1 homolog 2??[Similar Products]
UniProt Entry Name
ESCO2_MOUSE
UniProt Comments for ESCO2
ESCO2: Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during the S phase. Acetylates the cohesin component SMC3. Defects in ESCO2 are the cause of Roberts syndrome (RBS). RBS is a rare autosomal recessive disorder characterized by pre- and postnatal growth retardation, microcephaly, bilateral cleft lip and palate, and mesomelic symmetric limb reduction. Severely affected infants may be stillborn or die shortly after birth. RBS chromosomes have a lack of cohesion involving the heterochromatic C-banding regions around centromeres and the distal portion of the long arm of the Y chromosome (known as premature centromere separation, heterochromatin repulsion or puffing, or RS effect). Defects in ESCO2 are the cause of SC phocomelia syndrome (SCPS); also known as SC pseudothalidomide syndrome. SCPS has a milder phenotype than RBS, with a lesser degree of symmetric limb reduction and additionally includes flexion contractures of various joints, midfacial hemangioma, hypoplastic cartilage of ears and nose, scant silvery-blond hair, and cloudy corneae. Although microcephaly is present, mental retardation may be mild and survival into *****hood is common. Belongs to the acetyltransferase family. GCN5 subfamily.
Protein type: Acetyltransferase; DNA replication; EC 2.3.1.-
Cellular Component: XY body; Golgi apparatus; chromosome; nucleus; chromatin; chromocenter
Molecular Function: lysine N-acetyltransferase activity; keto acid formate lyase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; metal ion binding; 3-hydroxybutyryl-CoA thiolase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; acyl-CoA N-acyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; succinyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; C-acyltransferase activity; C-palmitoyltransferase activity; dihydrolipoamide S-acyltransferase activity; N-acetyltransferase activity; protein-cysteine S-acyltransferase activity; serine O-acyltransferase activity; malonyltransferase activity; octanoyltransferase activity; O-palmitoyltransferase activity; O-succinyltransferase activity; carnitine O-acyltransferase activity; sterol O-acyltransferase activity; 3-ketopimelyl-CoA thiolase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; N-palmitoyltransferase activity; O-acyltransferase activity; acylglycerol O-acyltransferase activity; O-acetyltransferase activity; benzoyl acetate-CoA thiolase activity; transferase activity; sinapoyltransferase activity; protein-cysteine S-myristoyltransferase activity; S-acyltransferase activity; acetyltransferase activity; N-succinyltransferase activity; myristoyltransferase activity; transferase activity, transferring acyl groups; L-2-aminoadipate N-acetyltransferase activity
Biological Process: double-strand break repair; hemopoietic progenitor cell differentiation; cell cycle; chromosome segregation; regulation of DNA replication
Research Articles on ESCO2
1. To Roberts syndrome(RBS), mouse Esco2 turns out to be a cell viability factor, the absence of which results in severe chromosome segregation defects and apoptosis.
Precautions
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