For Research Use Only. Not for use in diagnostic procedures.

Affinity Purified - Liquid

Store at 4-8 degrees

Small volumes of anti-5HT5A R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The histochemical antibody for 5-HT5A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor.

The antibody produces 4+ (maximum) labeling of 5-HT5A receptor at dilutions of 1/100 - 1/300 using both indirect immunofluorescence and biotin-streptavidin/HRP technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Antibodies; 5HT-Serotonins; 5HT (Serotonin) 5A Receptor; 5HT5A R

Immunohistochemistry (IHC), Western Blot (WB)

43,940 Da

5-hydroxytryptamine receptor 2A

5-hydroxytryptamine receptor 2A

5-hydroxytryptamine receptor 2A

HTR2; 5-HT-2; 5-HT-2A??[Similar Products]

5HT2A_HUMAN

5-HT(2A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 13q14-q21

Cellular Component: dendrite; plasma membrane

Molecular Function: drug binding; neurotransmitter receptor activity; serotonin binding; serotonin receptor activity

Biological Process: cellular calcium ion homeostasis; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); phosphoinositide 3-kinase cascade; phospholipase C activation; positive regulation of phosphatidylinositol biosynthetic process; release of sequestered calcium ion into cytosol; response to drug; serotonin receptor signaling pathway; synaptic transmission

Disease: Alcohol Dependence; Anorexia Nervosa, Susceptibility To, 1; Major Depressive Disorder; Obsessive-compulsive Disorder; Schizophrenia

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.