Full Product Name
GCK Polyclonal Antibody
Product Synonym Names
ATP:D-hexose 6-phosphotransferase; FGQTL3; GCK; GK; GLK; Glucokinase; Hexokinase D pancreatic isozyme; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HHF3; HK IV; HK4; HKIV; HXK4; HXKP; LGLK; MODY2
Product Gene Name
anti-GCK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35557
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration
1 mg/mL (lot specific)
Immunogen
Recombinant protein of human GCK
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GCK antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Product Categories/Family for anti-GCK antibody
Cancer; Metabolism; Signal Transduction
Applications Tested/Suitable for anti-GCK antibody
Immunofluorescence (IF)
Application Notes for anti-GCK antibody
IF: 1:20-1:100
Immunofluorescence (IF) of anti-GCK antibody
Immunofluorescence analysis of HeLa cells using GCK antibody.
NCBI/Uniprot data below describe general gene information for GCK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000153.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000162.5
[Other Products]
UniProt Primary Accession #
P35557
[Other Products]
UniProt Secondary Accession #
Q05810; A4D2J2; A4D2J3[Other Products]
UniProt Related Accession #
P35557[Other Products]
NCBI Official Full Name
glucokinase isoform 1
NCBI Official Synonym Full Names
glucokinase
NCBI Official Symbol
GCK??[Similar Products]
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
??[Similar Products]
NCBI Protein Information
glucokinase
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Protein Family
Glycerate 2-kinase
UniProt Gene Name
GCK??[Similar Products]
UniProt Synonym Gene Names
HK IV; HK4??[Similar Products]
NCBI Summary for GCK
This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]
UniProt Comments for GCK
Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
Research Articles on GCK
1. Our results suggest that the polymorphism of CYP3A4 *18B and GCK G-30A might affect the new-onset diabetes after transplantation (NODAT) development under tacrolimus-based immunotherapy. We suspect that the depression of GCK function may be a crucial pathogenic factor of tacrolimus-induced NODAT in variant carrier with A allele of GCK, but not be simply reflecting of glucose change.
Precautions
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Disclaimer
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