A-LAP; APPILS; ARTS-1; Adipocyte-derived leucine aminopeptidase precursor; Aminopeptidase PILS

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total ARTS-1 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-ARTS-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.Hattori A., J. Biochem. 130:235-241(2001).Nakajima D., DNA Res. 9:99-106(2002).Clark H.F., Genome Res. 13:2265-2270(2003).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from HepG2 cells, Jurkat cells and HUVEC cells, using ARTS-1 antibody.

107,841 Da

Endoplasmic reticulum aminopeptidase 1

APPILS; ARTS1; KIAA0525; A-LAP; PILS-AP??[Similar Products]

ERAP1_HUMAN

ARTS1: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. Belongs to the peptidase M1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.11.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region; integral to membrane; cytosol

Molecular Function: protein binding; interleukin-6 receptor binding; metalloexopeptidase activity; interleukin-1, Type II receptor binding; zinc ion binding; aminopeptidase activity

Biological Process: fat cell differentiation; positive regulation of angiogenesis; antigen processing and presentation of peptide antigen via MHC class I; response to bacterium; regulation of blood pressure; membrane protein ectodomain proteolysis; angiogenesis; antigen processing and presentation of endogenous peptide antigen via MHC class I; regulation of innate immune response

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