Full Product Name
GCK siRNA (Human)
Product Synonym Names
Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Product Gene Name
GCK sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35557
Specificity
GCK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GCK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GCK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GCK sirna
siRNA to inhibit GCK expression using RNA interference
Applications Tested/Suitable for GCK sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GCK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000153.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000162.3
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UniProt Primary Accession #
P35557
[Other Products]
UniProt Secondary Accession #
Q05810; A4D2J2; A4D2J3[Other Products]
UniProt Related Accession #
P35557[Other Products]
Molecular Weight
52,035 Da
NCBI Official Full Name
glucokinase isoform 1
NCBI Official Synonym Full Names
glucokinase (hexokinase 4)
NCBI Official Symbol
GCK??[Similar Products]
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
??[Similar Products]
NCBI Protein Information
glucokinase
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Protein Family
Glycerate 2-kinase
UniProt Gene Name
GCK??[Similar Products]
UniProt Synonym Gene Names
HK IV; HK4??[Similar Products]
UniProt Entry Name
HXK4_HUMAN
NCBI Summary for GCK
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]
UniProt Comments for GCK
GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a ""glucose sensor"" in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described.
Protein type: Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Carbohydrate Metabolism - galactose; EC 2.7.1.2
Chromosomal Location of Human Ortholog: 7p15.3-p15.1
Cellular Component: nucleoplasm; mitochondrion; actin filament; cytosol; basal cortex; secretory granule
Molecular Function: protein binding; mannokinase activity; fructokinase activity; magnesium ion binding; ADP binding; glucokinase activity; protein phosphatase binding; ATP binding; glucose binding
Biological Process: positive regulation of glycogen biosynthetic process; glucose 6-phosphate metabolic process; lipid homeostasis; pathogenesis; glucose transport; glucose homeostasis; NADP metabolic process; cellular response to glucose starvation; detection of glucose; elevation of cytosolic calcium ion concentration; negative regulation of gluconeogenesis; regulation of glycolysis; transmembrane transport; second-messenger-mediated signaling; glycogen biosynthetic process; glycolysis; regulation of potassium ion transport; positive regulation of insulin secretion; glucose metabolic process; endocrine pancreas development; fructose 2,6-bisphosphate metabolic process; cellular response to insulin stimulus; hexose transport; carbohydrate phosphorylation; carbohydrate metabolic process; positive regulation of glycolysis; negative regulation of epinephrine secretion; cell glucose homeostasis; regulation of insulin secretion; positive regulation of phosphorylation
Disease: Hyperinsulinemic Hypoglycemia, Familial, 3; Diabetes Mellitus, Permanent Neonatal; Maturity-onset Diabetes Of The Young, Type 2; Diabetes Mellitus, Noninsulin-dependent
Research Articles on GCK
1. Two novel GCK splicing mutations in Maturity Onset Diabetes of Young 2 have been characterized.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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