Full Product Name
Anti-GFAP Antibody
Product Synonym Names
Glial fibrillary acidic protein; GFAP
Product Gene Name
anti-GFAP antibody
[Similar Products]
Antibody/Peptide Pairs
GFAP peptide (MBS8243557) is used for blocking the activity of GFAP antibody (MBS8242718)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P14136
Species Reactivity
Human, Bovine, Monkey, Sheep
Specificity
Recognizes endogenous levels of GFAP protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GFAP. The exact sequence is proprietary.
Preparation and Storage
Shipped and store at 4 degree C for one year. Do not freeze.
Other Notes
Small volumes of anti-GFAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GFAP antibody
Rabbit polyclonal antibody to GFAP
Applications Tested/Suitable for anti-GFAP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-GFAP antibody
Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
Western Blot (WB) of anti-GFAP antibody
Western blot analysis of GFAP expression in HeLa (A), mouse brain (B), rat muscle (C) whole cell lysates.
Immunofluorescence (IF) of anti-GFAP antibody
Immunofluorescent analysis of GFAP staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.
NCBI Accession #
P14136.1
[Other Products]
UniProt Primary Accession #
P14136
[Other Products]
UniProt Secondary Accession #
Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3[Other Products]
UniProt Related Accession #
P14136[Other Products]
Molecular Weight
49,508 Da
NCBI Official Full Name
Glial fibrillary acidic protein
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP??[Similar Products]
NCBI Official Synonym Symbols
ALXDRD
??[Similar Products]
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
Glial fibrillary acidic protein
UniProt Gene Name
GFAP??[Similar Products]
UniProt Synonym Gene Names
GFAP??[Similar Products]
UniProt Entry Name
GFAP_HUMAN
NCBI Summary for GFAP
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
UniProt Comments for GFAP
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: cytoplasm; cytosol; intermediate filament; intermediate filament cytoskeleton; lysosome; membrane; myelin sheath
Molecular Function: integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton
Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; intermediate filament organization; neurite regeneration; regulation of neurotransmitter uptake; regulation of protein complex assembly; response to wounding
Disease: Alexander Disease
Research Articles on GFAP
1. Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
