Full Product Name
MOUSE ANTI BOVINE GFAP
Product Gene Name
anti-GFAP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Chicken, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
Bovine spinal cord homogenate.
Histology Positive Control Tissue
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-GFAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GFAP antibody
Mouse anti Bovine GFAP antibody, clone 2E1 recognizes glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death. Mouse anti Bovine GFAP antibody, clone 2E1 may be used in conjunction with clones 1B4 and 4A11 for increased sensitivity when used in immunohistology.
Applications Tested/Suitable for anti-GFAP antibody
Immunohistology Frozen, Immunohistology Paraffin*, Radioimmunoassays (RIA), Western Blot (WB)
Application Notes for anti-GFAP antibody
Immunohistology - Paraffin: Minimum Dilution: 1/25; Maximum Dilution: 1/100; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Testing Data of anti-GFAP antibody
Paraffin embedded human brain stained with Mouse anti Bovine GFAP following pronase antigen retrieval
NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058705.2
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NCBI GenBank Nucleotide #
NM_017009.2
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UniProt Secondary Accession #
Q7TQ31; Q9R1Q3; Q9Z2S0[Other Products]
UniProt Related Accession #
P47819[Other Products]
Molecular Weight
48,782 Da
NCBI Official Full Name
glial fibrillary acidic protein
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
Gfap??[Similar Products]
NCBI Protein Information
glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
Glial fibrillary acidic protein
UniProt Gene Name
Gfap??[Similar Products]
UniProt Synonym Gene Names
GFAP??[Similar Products]
UniProt Entry Name
GFAP_RAT
NCBI Summary for GFAP
intermediate filament protein specific for astrocytes [RGD, Feb 2006]
UniProt Comments for GFAP
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Cellular Component: cell projection; membrane; cytoplasm; intermediate filament; myelin sheath
Molecular Function: integrin binding; structural constituent of cytoskeleton; structural molecule activity; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; intermediate filament-based process; astrocyte development
Research Articles on GFAP
1. Data indicate that histone demethylases KDM4A/C regulate H3K9 and H3K36 methylation on brain-derived neurotrophic factor (BDNF) versus astroglial glial fibrillary acidic protein (GFAP) genes in neural stem cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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