Rat 5-hydroxytryptamine receptor 1A (HTR1A) ELISA kit; 5-HT1A; 5HT1a; ADRB2RL1; ADRBRL1; 5-HT1a receptor; G protein coupled receptor; guanine nucleotide-binding regulatory protein-coupled receptor; 5-hydroxytryptamine (serotonin) receptor 1A

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of rat HTR1A. No significant cross-reactivity or interference between rat HTR1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HTR1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS928226 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR1A. The ELISA analytical biochemical technique of the MBS928226 kit is based on HTR1A antibody-HTR1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

46,429 Da

5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

5-hydroxytryptamine receptor 1A; 5-HT1A; 5-HT-1A; serotonin receptor 1A

5-hydroxytryptamine receptor 1A

5ht1a; 5-HT-1A; 5-HT1A??[Similar Products]

5HT1A_RAT

regulates AMPA receptor channels in the prefrontal cortex; regulation is mediated via inhibition of Ca2+/calmodulin-dependent kinase II [RGD, Feb 2006]

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: axon hillock; cell soma; membrane; integral to plasma membrane

Molecular Function: serotonin binding; protein binding; serotonin receptor activity; G-protein alpha-subunit binding; drug binding

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; vasoconstriction; positive regulation of microtubule depolymerization; behavioral fear response; serotonin receptor, adenylate cyclase inhibiting pathway; regulation of serotonin secretion; regulation of dopamine metabolic process; regulation of hormone secretion; serotonin metabolic process; serotonin receptor signaling pathway; regulation of behavior

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