Full Product Name
GCK Polyclonal Antibody
Product Synonym Names
GCK; GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2
Product Gene Name
anti-GCK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35557
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
A synthetic peptide of human GCK
Calculated Molecular Weight
52kDa
Preparation and Storage
Store at 4 degree C (regular) or -20 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-GCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GCK antibody
Germinal center kinase (GCK) is the founding member of the GCK family, a group of serine/threonine kinases homologous to the yeast Ste20 kinase. GCK is activated by TNF and associated with TRAF2 (TNF receptor-associated factor 2) and MEKK1, thereby activating the SAPK/JNK pathway (1,2). GCK does not significantly phosphorylate MEKK1, instead it enhances MEKK1 oligomerization and autophosphorylation (3). GCK binds and activates MLK3, indicating that GCK may have other effectors (3).
Product Categories/Family for anti-GCK antibody
Polyclonal
Applications Tested/Suitable for anti-GCK antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GCK antibody
WB: 1:200 - 1:500
IHC: 1:50 - 1:100
IF: 1:20 - 1:50
Western Blot (WB) of anti-GCK antibody
Western blot analysis of extracts of various cell lines, using GCK antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
NCBI/Uniprot data below describe general gene information for GCK. It may not necessarily be applicable to this product.
NCBI Accession #
P35557.1
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UniProt Primary Accession #
P35557
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UniProt Secondary Accession #
Q05810; A4D2J2; A4D2J3[Other Products]
UniProt Related Accession #
P35557[Other Products]
NCBI Official Full Name
Glucokinase
NCBI Official Synonym Full Names
glucokinase (hexokinase 4)
NCBI Official Symbol
GCK??[Similar Products]
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
??[Similar Products]
NCBI Protein Information
glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Protein Family
Glycerate 2-kinase
UniProt Gene Name
GCK??[Similar Products]
UniProt Synonym Gene Names
HK IV; HK4??[Similar Products]
UniProt Entry Name
HXK4_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
